Adrien Assié

Postdoctoral Researcher exploring host microbe interactions

I am fascinated by how two different organisms can evolve fine molecular mechanism in order to interact together, since my PhD dissecting metagenomes to find such apparatus has been my favorite thing to do.

What I Do
  • Bioinformatics
  • Metagenomes analysis
  • Transcriptomes analysis
  • Metabolic reconstructions
  • Phylogenies
  • Microscopy
  • F.I.S.H (Fluorescent in situ Hybridization)
  • Confocal microscopy
  • Molecular biology

 Skills

Bio informatics

Metagenomic  assembly and analysis.

Metatranscriptomic assembly and analysis.

Sample preparation for NGS sequencing

  • NGS raw sequences preparation
  • From the machine to the analysis pipeline
Phylogeny analysis

  • ARB, Mr Bayse

Molecular Biology

FISH : Fluorescence In Situ Hybridization

Microscopy : Epi- fluorescence and Confocal Laser Microscopy

Clone libraries preparation and analyses

PCR based studies

DNA extraction

 Publications

A. Assié, C. Borowski, K. van der Heijden, L. Raggi, N. Leisch, B. Geier, M. P. Schimak, N. Dubilier, J. M. Petersen. Environmental Microbiology Reports (2016) A specific and widespread association between deep-sea Bathymodiolus mussels and a novel family of Epsilonproteobacteria. doi:10.1111/1758-2229.12442

Talks and Posters

  • “It’s all about location! A study of bacterial ectosymbiotic communities of the deep sea shrimp Rimicaris hybisae.” Oral presentation – SilvaNGS workshop 2016
  • “An alternative pathway for inorganic carbon fixation in epsilonproteobacterial epibionts of deep-sea Bathymodiolus mussels”. Oral presentation – ISME 2016
  • “Diving into the genome of a deep-sea intranuclear bacteria”. Poster presentation – Young Researcher in Life Science conference 2014
  • “Novel epsilonproteobacterial epibionts on the gills of deep-sea mussels from hydrothermal vents and cold seeps”. Poster presentation – FEMS 2013
  • “Novel epsilonproteobacterial symbionts in gill tissues of deep-sea hydrothermal vent mussels”. Poster presentation – VAAM 2013

Awards

5th conference of the Young researcher in Life science

Best poster presentation – 3rd place – link

 Education

PhD Thesis

In marine microbiology - Deep sea symbiosis

Master thesis

In marine microbiology - Deep sea symbiosis

Max Planck Institute for Marine Microbiology 2012 – 2016

Department of Symbiosis
Germany – Bremen

“Biometry and evolutive biology laboratory”

CNRS-UMR5558
France – Lyon
Decembre 2010 – June 2011

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During my PhD thesis I studied different bacterial partners associated with deep sea invertebrate using different techniques like Fluorescent In Situ Hybridization (FISH); Next generation sequencing; Metagenomes and Metatranscriptome analysis.
My principal project focus on deep sea mussels belonging to the Bathymodiolus genus. These mussels are found worldwide at cold seeps and hydrothermal vents, they have been studied for decades for their unique association with gammaproteobacterial chemoautotroph which are settled in the host gill epithelia. Additionally to the main endosymbiont many Bathymodiolus species are associated with other low abundance bacterial phylotypes. My work was to characterize two of these secondary associated bacteria: an epibiotic epsilonproteobacteria and an intranuclear parasite.

A second part of my PhD thesis is the characterization of bacterial ectosymbiotic populations associated to a recently described  deep sea shrimp Rimicaris hybisae found on the Cayman Rise. These shrimps occur at two vent site located 20km and 2500m depth apart. These two vents fields are very distinct not only in depth but also in chemical composition which is the perfect setting to have a close look at the environment influence on ectosymbiotic populations compositions.
Funded by the Marie Curie International Training Network 7 (ITN)

The main topic of the master thesis was the study of mechanisms involved in host-endosymbiont interactions between Drosophila and the Alphaproteobacteria Wolbachia.
I have maintained lines of Drosophila melanosgaster, each one expressing an interfering RNA of a host gene. Once the line were set up I analyzed the impact of the host gene inactivation on the infecting Wolbachia population by quantifying a specific bacterial single copy gene with RT-qPCR.

Master in Microbiology

Infectiology: Microbiology, Virology and Immunology - Specialty: Fundamental Microbiology

Bachelor / Licence in Biology

Speciality in microbiology

Université Paris Diderot – Paris 7
France · Paris
September 2010 – June 2011
Université de Montpellier 2
Speciality in Microbiology
France · Montpellier
September 2007 – June 2010